canine population structure assessment and impact of intra-breed stratification on snp-based association studies犬种群结构评估和intra-breed分层snp-based协会研究的影响.pdfVIP

Canine Population Structure: Assessment and Impact of Intra-Breed Stratification on SNP-Based Association Studies 1 2 1 3 ´ 2 1 2 ´2 Pascale Quignon , Laetitia Herbin , Edouard Cadieu , Ewen F. Kirkness , Benoit Hedan , Dana S. Mosher , Francis Galibert , Catherine Andre , 1 2 Elaine A. Ostrander *, Christophe Hitte * 1 Cancer Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, United States of America, ´ ´ ´ ´ 2 CNRS UMR6061 Genetique et Developpement, Universite de Rennes 1, IFR140, CS 34317, Rennes, France, 3 The J Craig Venter Institute, Rockville, Maryland, United States of America Background. In canine genetics, the impact of population structure on whole genome association studies is typically addressed by sampling approximately equal numbers of cases and controls from dogs of a single breed, usually from the same country or geographic area. However one way to increase the power of genetic studies is to sample individuals of the same breed but from different geographic areas, with the expectation that independent meiotic events will have shortened the presumed ancestral haplotype around the mutation differently. Little is known, however, about genetic variation among dogs of the same breed collected from different geographic regions. Methodology/Principal Findings. In this report, we address the magnitude and impact of genetic diversity among common breeds sampled in the U.S. and Europe. The breeds selected, including the Rottweiler, Bernese mountain dog, flat-coated retriever, and golden retriever, share susceptibility to a class of soft tissue cancers typified by malignan