circadian rhythmicity by autocatalysis昼夜节律性的自动催化作用.pdfVIP

Circadian Rhythmicity by Autocatalysis 1 1,2 1 1,3 1 2* Arun Mehra , Christian I. Hong , Mi Shi , Jennifer J. Loros , Jay C. Dunlap , Peter Ruoff 1 Department of Genetics, Dartmouth Medical School, Hanover, New Hampshire, United States of America, 2 Department of Mathematics and Natural Science, University of Stavanger, Stavanger, Norway, 3 Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire, United States of America The temperature compensated in vitro oscillation of cyanobacterial KaiC phosphorylation, the first example of a thermodynamically closed system showing circadian rhythmicity, only involves the three Kai proteins (KaiA, KaiB, and KaiC) and ATP. In this paper, we describe a model in which the KaiA- and KaiB-assisted autocatalytic phosphorylation and dephosphorylation of KaiC are the source for circadian rhythmicity. This model, based upon autocatalysis instead of transcription-translation negative feedback, shows temperature-compensated circadian limit-cycle oscillations with KaiC phosphorylation profiles and has period lengths and rate constant values that are consistent with experimental observations. Citation: Mehra A, Hong CI, Shi M, Loros JJ, Dunlap JC, et al. (2006) Circadian rhythmicity by autocatalysis. PLoS Comput Biol 2(7): e96. DOI: 10.1371/journal.pcbi.0020096 Introduction establishing a basic model based on known biological and biochemical observations that did not involve transcription Circadian rhythms are environmentally adaptive and or translation. In Figure 1, we summarize key steps that we homeostatically regulated oscillators found in many organ-