circadian regulation of food-anticipatory activity in molecular clock–deficient mice生理调节food-anticipatory活动分子clock-deficient老鼠.pdfVIP

Circadian Regulation of Food-Anticipatory Activity in Molecular Clock–Deficient Mice 1 2 3 2 4 Nana N. Takasu , Gen Kurosawa , Isao T. Tokuda , Atsushi Mochizuki , Takeshi Todo , Wataru Nakamura1,5* 1 Laboratory of Oral Chronobiology, Graduate School of Dentistry, Osaka University, Osaka, Japan, 2 Theoretical Biology Laboratory, RIKEN Advanced Science Institute, Wako, Japan, 3 Department of Micro System Technology, Ritsumeikan University, Shiga, Japan, 4 Department of Radiation Biology and Medical Genetics, Graduate School of Medicine, Osaka University, Osaka, Japan, 5 Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Kawaguchi, Japan Abstract In the mammalian brain, the suprachiasmatic nucleus (SCN) of the anterior hypothalamus is considered to be the principal circadian pacemaker, keeping the rhythm of most physiological and behavioral processes on the basis of light/dark cycles. Because restriction of food availability to a certain time of day elicits anticipatory behavior even after ablation of the SCN, such behavior has been assumed to be under the control of another circadian oscillator. According to recent studies, however, mutant mice lacking circadian clock function exhibit normal food-anticipatory activity (FAA), a daily increase in locomotor activity preceding periodic feeding, suggesting that FAA is independent of the known circadian oscillator. To investigate the molecular basis of FAA, we examined oscillatory properties in mice lacking molecular clock components. Mice with SCN lesions or with mutant circadian periods were exposed to restricted feeding schedules at periods within and outside circadian range. Periodic feeding led to the entrainment of FAA rhythms only within a limi