comparative expression profiles of midgut genes in dengue virus refractory and susceptible aedes aegypti across critical period for virus infection比较基因表达谱中肠的登革病毒耐火材料和敏感埃及伊蚊在病毒感染的关键时期.pdfVIP

Comparative Expression Profiles of Midgut Genes in Dengue Virus Refractory and Susceptible Aedes aegypti across Critical Period for Virus Infection Chitra Chauhan.{, Susanta K. Behura., Becky deBruyn, Diane D. Lovin, Brent W. Harker, Consuelo Gomez-Machorro, Akio Mori, Jeanne Romero-Severson, David W. Severson* Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana, United States of America Abstract Background: Aedes aegypti is the primary mosquito vector for dengue virus (DENV) worldwide. Infectivity of dengue virus varies among natural populations of this mosquito. How A. aegypti responds to DENV infection relative to which genes and associated pathways contribute to its differential susceptibility as a vector is not well defined. Methods/Principal Findings: Here, we used custom cDNA microarrays to identify groups of genes that were differentially expressed in midgut tissues between susceptible and refractory strains in a highly time specific manner. While genes involved in protein processing in the endoplasmic reticulum, mRNA surveillance, and the proteasome were significantly up- regulated in the susceptible strain, several metabolic processes including glycolysis, glycan biosynthesis and Wnt pathway were active in the refractory strain. In addition, several key signaling genes were expressed as common responsive genes in both susceptible and refractory mosquitoes that may be necessary for signal transduction to trigger the appropriate host response to the viral infection. These are coordinately expressed in the form of tight gene networks and expression clusters that may be necessary to differentially contribute to the progression of dengue infection between the two strains. Conclusions: Our data show that highly correlated differential expression of responsive genes throughout the post infection