comparative expression profiling of leishmania modulation in gene expression between species and in different host genetic backgrounds利什曼虫的比较表达式分析调制在不同物种之间的基因表达和宿主遗传背景.pdfVIP

Comparative Expression Profiling of Leishmania: Modulation in Gene Expression between Species and in Different Host Genetic Backgrounds 1 1 2 3 1 1 Daniel P. Depledge , Krystal J. Evans , Alasdair C. Ivens , Naveed Aziz , Asher Maroof , Paul M. Kaye , Deborah F. Smith1* 1 Centre for Immunology and Infection, Department of Biology/Hull York Medical School, University of York, York, United Kingdom, 2 Fios Genomics, ETTC, King’s Buildings, Edinburgh, United Kingdom, 3 Technology Facility, Department of Biology, University of York, York, United Kingdom Abstract Background: Genome sequencing of Leishmania species that give rise to a range of disease phenotypes in the host has revealed highly conserved gene content and synteny across the genus. Only a small number of genes are differentially distributed between the three species sequenced to date, L. major, L. infantum and L. braziliensis. It is not yet known how many of these genes are expressed in the disease-promoting intracellular amastigotes of these species or whether genes conserved between the species are differentially expressed in the host. Methods/Principal Findings: We have used customised oligonucleotide microarrays to confirm that all of the differentially distributed genes identified by genome comparisons are expressed in intracellular amastigotes, with only a few of these subject to regulation at the RNA level. In the first large-scale study of gene expression in L. braziliensis, we show that only ,9% of the genes analysed are regulated in their RNA expression during the L. braziliensis life cycle, a figure consistent with that observed in other Leishmania species. Comparing amastigote gene expression profiles between species confirms the