comparative pathogenesis of an avian h5n2 and a swine h1n1 influenza virus in pigs比较禽流感的发病机理h5n2和h1n1猪流感病毒在猪身上.pdfVIP

Comparative Pathogenesis of an Avian H5N2 and a Swine H1N1 Influenza Virus in Pigs 1 1 2 2 Annebel De Vleeschauwer , Kalina Atanasova , Steven Van Borm , Thierry van den Berg , Thomas 3 ˚ 3 1 Bruun Rasmussen , Ase Uttenthal , Kristien Van Reeth * 1 Laboratory of Virology, Faculty of Veterinary Medicine Ghent University, Merelbeke, Belgium, 2 Avian Virology Immunology, Veterinary Agrochemical Research Centre, Brussels, Belgium, 3 National Veterinary Institute, Technical University of Denmark, Kalvehave, Denmark Abstract Pigs are considered intermediate hosts for the transmission of avian influenza viruses (AIVs) to humans but the basic organ pathogenesis of AIVs in pigs has been barely studied. We have used 42 four-week-old influenza naive pigs and two different inoculation routes (intranasal and intratracheal) to compare the pathogenesis of a low pathogenic (LP) H5N2 AIV with that of an H1N1 swine influenza virus. The respiratory tract and selected extra-respiratory tissues were examined for virus replication by titration, immunofluorescence and RT-PCR throughout the course of infection. Both viruses caused a productive infection of the entire respiratory tract and epithelial cells in the lungs were the major target. Compared to the swine virus, the AIV produced lower virus titers and fewer antigen positive cells at all levels of the respiratory tract. The respiratory part of the nasal mucosa in particular showed only rare AIV positive cells and this was associated with reduced nasal shedding of the avian compared to the swine virus. The titers and distribution of the AIV varied extremely between indivi