circulating mir-17, mir-20a, mir-29c, and mir-223 combined as non-invasive biomarkers in nasopharyngeal carcinoma循环mir - 17、mir-20a mir-29c,mir - 223结合鼻咽癌的非侵入性的生物标志物.pdfVIP

Circulating miR-17, miR-20a, miR-29c, and miR-223 Combined as Non-Invasive Biomarkers in Nasopharyngeal Carcinoma 1,2 1 1 1 1,2 1,2 3 Xi Zeng , Juanjuan Xiang , Minghua Wu , Wei Xiong , Hailin Tang , Min Deng , Xiayu Li , 1,2 1,2 1 1 1 1 1 Qianjin Liao , Bo Su , Zhaohui Luo , Yanhong Zhou , Ming Zhou , Zhaoyang Zeng , Xiaoling Li , 3 4 1 5 1 Shourong Shen , Cijun Shuai , Guiyuan Li , Jiasheng Fang *, Shuping Peng * 1 Cancer Research Institute, Central South University, Changsha, Hunan, P.R. China, 2 Cancer Research Institute, University of South China, Hengyang, Hunan, P.R. China, 3 Third Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China, 4 State Key Laboratory of High Performance Complex Manufacturing, Central South University, Changsha, Hunan, P.R. China, 5 Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China Abstract Background: MicroRNAs have been considered as a kind of potential novel biomarker for cancer detection due to their remarkable stability in the blood and the characteristics of their expression profile in many diseases. Methods: We performed microarray-based serum miRNA profiling on the serum of twenty nasopharyngeal carcinoma patients at diagnosis along with 20 non-cancerous individuals as controls. This was followed by a real-time quantitative Polymerase Chain Reaction (RT-qPCR) in a separate cohort of thirty patients with nasopharyngeal carcinoma an