comparative performance of electrochemiluminescence immunoassay and eia for hiv screening in a multiethnic region of china比较性能的电化学发光免疫分析和环境影响评价在中国多民族地区的艾滋病筛查.pdfVIP

Comparative Performance of Electrochemiluminescence Immunoassay and EIA for HIV Screening in a Multiethnic Region of China 1 1 1 1 1 2 Xiaohui Bi , Hongxia Ning , Tingting Wang , Dongdong Li , Yongming Liu , Tingfu Yang , 2 2 Jiansheng Yu , Chuanmin Tao * 1 Division of Clinical Microbiology, West China Hospital, Sichuan University, Chengdu, China, 2 Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China Abstract Background: The recent approval of 4th generation HIV tests has forced many laboratories to decide whether to shift from 3rd to these tests. There are limited published studies on the comparative evaluation of these two different assays. We compare the performance of fourth-generation electrochemiluminescence immunoassay (ChIA) and third-generation enzyme linked immunosorbent assay (EIA) for human immunodeficiency virus (HIV) screening and gauge whether the shift from EIA to ChIA could be better in a multiethnic region of China. Methodology/Principal Findings: We identified a large number of routine specimens (345,492) using two different assays from Jan 2008 to Aug 2011 in a teaching hospital with high sample throughput. Of the 344,596 specimens with interpretable HIV test results, 526(0.23%) of 228,761 using EIA and 303(0.26%) of 115,835 using ChIA were HIV-1 positive. The false-positive rate of EIA was lower than that of ChIA [0.03% vs. 0.08%, odds ratio 0.33 (95% confidence interval 0.24, 0.45)]. The positive predictive value (PPV) of EIA (89.6%) was significantly higher than that of ChIA (76.1%) (,0.001), reflecting the difference between