comparative proteomics analyses reveal the virb of b. melitensis affects expression of intracellular survival related proteins比较蛋白质组学分析揭示了b的virb melitensis影响细胞内生存相关蛋白的表达.pdfVIP

Comparative Proteomics Analyses Reveal the virB of B. melitensis Affects Expression of Intracellular Survival Related Proteins 1. 1. 1 3 1 1 2 Yufei Wang , Zeliang Chen *, Feng Qiao , Tianyi Ying , Jing Yuan , Zhijun Zhong , Lei Zhou , Xinying 1 1 1 1 1 1 2 1 Du , Zhoujia Wang , Jin Zhao , Shicun Dong , Leili Jia , Xitong Yuan , Ruifu Yang , Yansong Sun , Liuyu Huang1* 1 Institute of Disease Control and Prevention, Academy of Military Medical Science, Beijing, China, 2 Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing, China, 3 Beijing Institute of Pharmaceutical Chemistry, Beijing, China Abstract Backgound: Brucella melitensis is a facultative, intracellular, pathogenic bacterium that replicates within macrophages. The type IV secretion system encoded by the virB operon (virB) is involved in Brucella intracellular survival. However, the underlying molecular mechanisms, especially the target proteins affected by the virB, remain largely unclear. Methodology/Principal Findings: In order to define the proteins affected by virB, the proteomes of wild-type and the virB mutant were compared under in vitro conditions where virB was highly activated. The differentially expressed proteins were identified by MALDI-TOF-MS. Forty-four down-regulated and eighteen up-regulated proteins which exhibited a 2-fold or greater change were identified. These proteins included those involved in amino acid transport and metabolism, lipid metabolism, energy production, cell membrane biogenesis, translation, post-translational mod