comprehensive dissection of pdgf-pdgfr signaling pathways in pdgfr genetically defined cells全面解剖pdgf-pdgfr pdgfr基因定义的细胞信号通路.pdfVIP

Comprehensive Dissection of PDGF-PDGFR Signaling Pathways in PDGFR Genetically Defined Cells 1,2. 3. 1 1 1 1 Erxi Wu , Nathan Palmer , Ze Tian , Annie P. Moseman , Michal Galdzicki , Xuetao Wang , Bonnie 3,4 5 1,2 Berger , Hongbing Zhang , Isaac S. Kohane * 1 Informatics Program, Children’s Hospital Boston, Harvard Medical School, Boston, Massachusetts, United States of America, 2 Division of Health Sciences and Technology, Harvard University and Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America, 3 Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America, 4 Department of Mathematics, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America, 5 Department of Physiology Pathophysiology, National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Tsinghua University, Beijing, China Abstract Despite the growing understanding of PDGF signaling, studies of PDGF function have encountered two major obstacles: the functional redundancy of PDGFRa and PDGFRb in vitro and their distinct roles in vivo. Here we used wild-type mouse embryonic fibroblasts (MEF), MEF null for either PDGFRa, b, or both to dissect PDGF-PDGFR signaling pathways. These four PDGFR genetically defined cells provided us a platform to study the relative contributions of the pathways triggered by the two PDGF receptors. They were treated with PDGF-BB and analyzed for differential gene expression, in