core proteome of the minimal cell comparative proteomics of three mollicute species最小的细胞比较蛋白质组学的核心蛋白质组的三个mollicute物种.pdfVIP

Core Proteome of the Minimal Cell: Comparative Proteomics of Three Mollicute Species 1 1,3 1 1 Gleb Y. Fisunov *, Dmitry G. Alexeev , Nicolay A. Bazaleev , Valentina G. Ladygina , Maria A. 1 1 2 1 1 Galyamina , Ilya G. Kondratov , Nadezhda A. Zhukova , Marina V. Serebryakova , Irina A. Demina , Vadim M. Govorun1,2,3 1 Scientific Research Institute of Physical-Chemical Medicine, Federal Bio-Medical Agency of Russia, Moscow, Russia, 2 Russian Research Centre Kurchatov Institute, Moscow, Russia, 3 Moscow Institute of Physics and Technology, Moscow, Russia Abstract Mollicutes (mycoplasmas) have been recognized as highly evolved prokaryotes with an extremely small genome size and very limited coding capacity. Thus, they may serve as a model of a ‘minimal cell’: a cell with the lowest possible number of genes yet capable of autonomous self-replication. We present the results of a comparative analysis of proteomes of three mycoplasma species: A. laidlawii, M. gallisepticum, and M. mobile. The core proteome components found in the three mycoplasma species are involved in fundamental cellular processes which are necessary for the free living of cells. They include replication, transcription, translation, and minimal metabolism. The members of the proteome core seem to be tightly interconnected with a number of interactions forming core interactome whether or not additional species-specific proteins are located on the periphery. We also obtained a genome core of the respective organisms and compared it with the proteome core. It was found that the genome core e