comparative mirna expression profiles in individuals with latent and active tuberculosis比较microrna的表达谱在潜伏性和活动性肺结核患者.pdfVIP

Comparative miRNA Expression Profiles in Individuals with Latent and Active Tuberculosis 1. 1. 1 2 2 3 1 Chuan Wang , Shunyao Yang , Gang Sun , Xuying Tang , Shuihua Lu , Olivier Neyrolles , Qian Gao * 1 Key Laboratory of Medical Molecular Virology and Institute of Biomedical Sciences, Fudan University, Shanghai, China, 2 Respiratory Branch, Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai, China, 3 Institut de Pharmacologie et de Biologie Structurale, Centre National de la Recherche Scientifique and ´ Universite de Toulouse, Toulouse, France Abstract The mechanism of latent tuberculosis (TB) infection remains elusive. Several host factors that are involved in this complex process were previously identified. Micro RNAs (miRNAs) are endogenous ,22 nt RNAs that play important regulatory roles in a wide range of biological processes. Several studies demonstrated the clinical usefulness of miRNAs as diagnostic or prognostic biomarkers in various malignancies and in a few nonmalignant diseases. To study the role of miRNAs in the transition from latent to active TB and to discover candidate biomarkers of this transition, we used human miRNA microarrays to probe the transcriptome of peripheral blood mononuclear cells (PBMCs) in patients with active TB, latent TB infection (LTBI), and healthy controls. Using the software package BRB Array Tools for data analyses, 17 miRNAs were differentially expressed between the three groups (P,0.01). Hierarchical clustering of the 17 miRNAs expression profiles showed that individuals with active TB clustered independently of individuals with LTBI or from healthy controls.