原创力文档cryopreserved reticulocytes derived from hematopoietic stem cells can be invaded by cryopreserved plasmodium vivax isolates低温贮藏网织红细胞来源于造血干细胞可以通过低温贮藏间日疟原虫入侵隔离.pdfVIP
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cryopreserved reticulocytes derived from hematopoietic stem cells can be invaded by cryopreserved plasmodium vivax isolates低温贮藏网织红细胞来源于造血干细胞可以通过低温贮藏间日疟原虫入侵隔离
Cryopreserved Reticulocytes Derived from
Hematopoietic Stem Cells Can Be Invaded by
Cryopreserved Plasmodium vivax Isolates
1,3 1 1 2 3
*
´
Florian Noulin , Celine Borlon , Peter van den Eede , Luc Boel , Catherine M. Verfaillie ,
Umberto D’Alessandro1,4, Annette Erhart1
1 Unit of Malariology, Institute of Tropical Medicine Antwerp, Belgium, 2 Unit of Immunology, Institute of Tropical Medicine Antwerp, Belgium, 3 Stem cells institute,
Catholic University Leuven, Belgium, 4 Medical Research Council Unit, Fajara, The Gambia
Abstract
The development of a system for the continuous culture of Plasmodium vivax in vitro would benefit from the use of
reticulocytes derived from differentiated hematopoietic stem cells (HCS). At present, the need to use both fresh
reticulocytes and fresh P. vivax isolates represents a major obstacle towards this goal, particularly for laboratories located in
non-endemic countries. Here, we describe a new method for the cryopreservation of HSC-derived reticulocytes to be used
for both P. falciparum and P. vivax invasion tests. Cryopreserved P. falciparum and P. vivax isolates could invade both fresh
and cryopreserved HSC-derived reticulocytes with similar efficiency. This new technique allows the storage of HSC-derived
reticulocytes which can be used for later invasion tests and represents an important step towards the establishment of
a continuous P. vivax culture.
Citation: Noulin F, Borlon C, van den Eede P, Boel L, Verfaillie CM, et al. (2012) Cryopreserved Reticulocytes Derived from Hematopoietic Stem Cells Can Be
Invaded by Cryopreserved Plasmodium vivax Isolates. PLoS ONE 7(7): e40798. d
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