deletion of the viral anti-apoptotic gene f1l in the hivaids vaccine candidate mva-c enhances immune responses against hiv-1 antigens删除病毒抗凋亡基因f1l条件的候选疫苗mva-c提高针对hiv - 1抗原的免疫反应.pdfVIP

Deletion of the Viral Anti-Apoptotic Gene F1L in the HIV/ AIDS Vaccine Candidate MVA-C Enhances Immune Responses against HIV-1 Antigens 1. ´ 1. ´ 1 2 Beatriz Perdiguero , Carmen Elena Gomez , Jose Luis Najera , Carlos Oscar S. Sorzano , 3 ´ ´ 1 ´ 1 3 3 Julie Delaloye , Ruben Gonzalez-Sanz , Victoria Jimenez , Thierry Roger , Thierry Calandra , 4 1 Giuseppe Pantaleo , Mariano Esteban * ´ ´ 1 Department of Molecular and Cellular Biology, Centro Nacional de Biotecnologıa, Consejo Superior de Investigaciones Cientıficas (CSIC), Madrid, Spain, 2 Biocomputing ´ ´ Unit, Centro Nacional de Biotecnologıa, Consejo Superior de Investigaciones Cientıficas (CSIC), Madrid, Spain, 3 Infectious Diseases Service, Department of Medicine, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland, 4 Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland Abstract Vaccinia virus (VACV) encodes an anti-apoptotic Bcl-2-like protein F1 that acts as an inhibitor of caspase-9 and of the Bak/ Bax checkpoint but the role of this gene in immune responses is not known. Because dendritic cells that have phagocytosed apoptotic infected cells cross-present viral antigens to cytotoxic T cells inducing an antigen-specific immunity, we hypot