depletion of regulatory t lymphocytes reverses the imbalance between pro- and anti-tumor immunities via enhancing antigen-specific t cell immune responses损耗的调节性t淋巴细胞逆转职业之间的不平衡,通过增强抗肿瘤免疫力抗原t细胞免疫反应.pdfVIP

Depletion of Regulatory T Lymphocytes Reverses the Imbalance between Pro- and Anti-Tumor Immunities via Enhancing Antigen-Specific T Cell Immune Responses 1,2,3 4,6 3 4 3,4,5 Yu-Li Chen , Ming-Cheng Chang , Chi-An Chen , Han-Wei Lin , Wen-Fang Cheng *, Chung- Liang Chien1* 1 Graduate Institute of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, Taiwan, 2 Gynecologic Cancer Center, Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei, Taiwan, 3 Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University, Taipei, Taiwan, 4 Graduate Institute of Oncology, College of Medicine, National Taiwan University, Taipei, Taiwan, 5 Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan, 6 Department of Anesthesiology, College of Medicine, National Taiwan University, Taipei, Taiwan Abstract Background: The regulatory T cells (Tregs) can actively suppress the immune responses. However, literature about detailed changes of host effective and suppressive immunities before and after depletion of Tregs in ovarian carcinomas, is rare. Materials and Methods: Ovarian cancer patients and the ascitogenic animal model were employed. Immunologic profiles with flow cytometric analyses, immunohistochemistric staining, RT-PCR, ELISA, and ELISPOT assays were performed. In vivo depletion of Treg cells with the mAb PC61was also performed in the animal model. Results: The cytokines, including IL-4 (p = 0.017) and TNF-a (p = 0.046), significantly decreased while others such as TGF-b (p = 0.013), IL-6 (p = 0.016), and IL-10 (p = 0.018) were elevated in ascites of ovarian cancer patients, when the disease progresse