depletion of murine intestinal microbiota effects on gut mucosa and epithelial gene expression耗竭小鼠肠道菌群的影响肠道粘膜上皮基因表达.pdfVIP

Depletion of Murine Intestinal Microbiota: Effects on Gut Mucosa and Epithelial Gene Expression 1 . 1. 1 1 1,2 Dag Henrik Reikvam * , Alexander Erofeev , Anders Sandvik , Vedrana Grcic , Frode Lars Jahnsen , 3 4 4 5,6 Peter Gaustad , Kathy D. McCoy , Andrew J. Macpherson , Leonardo A. Meza-Zepeda , Finn-Eirik Johansen1,2 1 Department of Pathology and Centre for Immune Regulation, University of Oslo, Oslo, Norway, 2 Department of Pathology, Oslo University Hospital Rikshospitalet, Oslo, ¨ Norway, 3 Institute of Microbiology, University of Oslo, Oslo, Norway, 4 Maurice Muller Laboratories, Department of Clinical Research, University of Bern, Bern, Switzerland, 5 Department of Tumor Biology, the Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway, 6 Norwegian Microarray Consortium, Department of Molecular Biosciences, University of Oslo, Oslo, Norway Abstract Background: Inappropriate cross talk between mammals and their gut microbiota may trigger intestinal inflammation and drive extra-intestinal immune-mediated diseases. Epithelial cells constitute the interface between gut microbiota and host tissue, and may regulate host responses to commensal enteric bacteria. Gnotobiotic animals represent a powerful approach to study bacterial-host interaction but are not readily accessible to the wide scientific community. We aimed at refining a protocol that in a robust manner would deplete the cultivable intestinal microb