determination of baylisascaris schroederi infection in wild giant pandas by an accurate and sensitive pcrce-sscp method测定baylisascaris schroederi感染野生大熊猫的准确和敏感pcrce-sscp方法.pdfVIP

Determination of Baylisascaris schroederi Infection in Wild Giant Pandas by an Accurate and Sensitive PCR/CE- SSCP Method 1 1 2 3 1 4 Wenping Zhang , Shangmian Yie , Bisong Yue *, Jielong Zhou , Renxiong An , Jiangdong Yang , 5 1 1 1 4 1 Wangli Chen , Chengdong Wang , Liang Zhang , Fujun Shen , Guangyou Yang , Rong Hou , Zhihe Zhang1* 1The Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, People’s Republic of China, 2 The Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Science, Sichuan University, Chengdu, People’s Republic of China, 3 College of Life Sciences, Southwest Forestry University, Kunming, People’s Republic of China, 4 College of Animal Science, Sichuan Agricultural University, Ya’an, People’s Republic of China, 5 Tangjiahe National Nature Reserve, Guangyuan, People’s Republic of China Abstract It has been recognized that other than habitat loss, degradation and fragmentation, the infection of the roundworm Baylisascaris schroederi (B. schroederi) is one of the major causes of death in wild giant pandas. However, the prevalence and intensity of the parasite infection has been inconsistently reported through a method that uses sedimentation-floatation followed by a microscope examination. This method fails to accurately determine infection because there are many bamboo residues and/or few B. schroederi eggs in the examined fecal samples. In the present study, we adopted a method that uses PCR and capillary electrophoresis combined with a single-strand conf