developmental characterization of the microrna-specific c. elegans argonautes alg-1 and alg-2microrna-specific秀丽隐杆线虫的发育特征阿尔戈号的船员alg-1 alg-2.pdfVIP

Developmental Characterization of the MicroRNA- Specific C. elegans Argonautes alg-1 and alg-2 1 1 1,2,3 1,4 Alejandro Vasquez-Rifo , Guillaume Jannot , Javier Armisen , Michel Labouesse , Syed Irfan 1 1 1,2,3 1 Ahmad Bukhari , Evelyne L. Rondeau , Eric A. Miska , Martin J. Simard * ˆ ´ ´ 1 Laval University Cancer Research Centre, Hotel-Dieu de Quebec (CHUQ), Quebec City, Quebec, Canada, 2 Wellcome Trust Cancer Research UK Gurdon Institute, The Henry Wellcome Building of Cancer and Developmental Biology, University of Cambridge, Cambridge, United Kingdom, 3 Department of Biochemistry, University of ´ Cambridge, Cambridge, United Kingdom, 4 Development and Stem Cells Program, IGBMC, CNRS (UMR7104), INSERM (U964), Universite de Strasbourg, BP10142, Illkirch, France Abstract The genes alg-1 and alg-2 (referred to as ‘‘alg-1/2’’) encode the Argonaute proteins affiliated to the microRNA (miRNA) pathway in C. elegans. Bound to miRNAs they form the effector complex that effects post-transcriptional gene silencing. In order to define biological features important to understand the mode of action of these Argonautes, we characterize aspects of these genes during development. We establish that alg-1/2 display an overlapping spatio-temporal expression profile and shared association to a miRNAs set, but with gene-specific pre