deletion of nhlh2 results in a defective torpor response and reduced beta adrenergic receptor expression in adipose tissue删除nhlh2导致有缺陷的迟钝反应,减少β肾上腺素能受体表达在脂肪组织中.pdfVIP

Deletion of Nhlh2 Results in a Defective Torpor Response and Reduced Beta Adrenergic Receptor Expression in Adipose Tissue 1 2 1,2 1,2 Umesh D. Wankhade , Kristen R. Vella , Dana L. Fox , Deborah J. Good * 1 Department of Human Nutrition, Foods and Exercise, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, United States of America, 2 Department of Veterinary and Animal Sciences and Molecular and Cellular Biology Graduate Program, University of Massachusetts, Amherst, Massachusetts, United States of America Abstract Background: Mice with a targeted deletion of the basic helix-loop-helix transcription factor, Nescient Helix-Loop-Helix 2 (Nhlh2), display adult-onset obesity with significant increases in their fat depots, abnormal responses to cold exposure, and reduced spontaneous physical activity levels. These phenotypes, accompanied by the hypothalamic expression of Nhlh2, make the Nhlh2 knockout (N2KO) mouse a useful model to study the role of central nervous system (CNS) control on peripheral tissue such as adipose tissue. Methodology: Differences in body temperature and serum analysis of leptin were performed in fasted and ad lib fed wild- type (WT) and N2KO mice. Histological analysis of white (WAT) and brown adipose tissue (BAT) was performed. Gene and protein level expression of inflammatory and metabolic markers were compared between the two genotypes. Principal Findings: We report significant differences in serum leptin levels and body temperature in N2KO mice compared with WT mice exposed to a 24-hour fast, suggestive of a defect in both white (WAT) and brown adipose tissue (BAT) function. As compared to WT mice, N2KO mice showed increased serum IL-6 protein and