diabetes alters intracellular calcium transients in cardiac endothelial cells糖尿病改变心脏内皮细胞胞内钙瞬变.pdfVIP

Diabetes Alters Intracellular Calcium Transients in Cardiac Endothelial Cells 1 1 2 3 3 1 Abdul Q. Sheikh , Jennifer R. Hurley , Wei Huang , Toloo Taghian , Andrei Kogan , Hongkwan Cho , 2 1 Yigang Wang , Daria A. Narmoneva * 1 School of Energy, Environmental, Biological and Medical Engineering, University of Cincinnati, Cincinnati, Ohio, United States of America, 2 Department of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, Cincinnati, Ohio, United States of America, 3 Physics Department, University of Cincinnati, Cincinnati, Ohio, United States of America Abstract Diabetic cardiomyopathy (DCM) is a diabetic complication, which results in myocardial dysfunction independent of other etiological factors. Abnormal intracellular calcium ([Ca2+] ) homeostasis has been implicated in DCM and may precede i clinical manifestation. Studies in cardiomyocytes have shown that diabetes results in impaired [Ca2+]i homeostasis due to altered sarcoplasmic reticulum Ca2+ ATPase (SERCA) and sodium-calcium exchanger (NCX) activity. Importantly, altered calcium homeostasis may also be involved in diabetes-associated endothelial dysfunction, including impaired endothelium- dependent relaxation and a diminished capacity to generate nitric oxide (NO), elevated cell adhesion molecules, and decreased angiogenic growth factors. However, the effect of diabetes on Ca2+ regulatory mechanisms in cardiac endothelial cells (CECs) remains unknown. The objective of this study was to determine the effect of diabetes on [Ca2+] homeostasis in