diabetic β-cells can achieve self-protection against oxidative stress through an adaptive up-regulation of their antioxidant defenses糖尿病β-cells可以实现自我保护对抗氧化应激通过一个自适应老年病的抗氧化防御系统.pdfVIP

Diabetic b-Cells Can Achieve Self-Protection against Oxidative Stress through an Adaptive Up-Regulation of Their Antioxidant Defenses ´ 1 1 1 2 1 3 Gregory Lacraz , Florence Figeac , Jamileh Movassat , Nadim Kassis , Josiane Coulaud , Anne Galinier , 3 ´ 1 1 1 Corinne Leloup , Danielle Bailbe , Franc¸oise Homo-Delarche , Bernard Portha * ´ ´ ´ 1 Equipe B2PE (Biologie et Pathologie du Pancreas Endocrine), Unite BFA (Biologie Fonctionnelle et Adaptative), Universite Paris-Diderot et CNRS EAC7059, Paris, France, ´ ´ ´ 2 Equipe HERGE, Unite BFA (Biologie Fonctionnelle et Adaptative), Universite Paris-Diderot et CNRS EAC7059, Paris, France, 3 UMR 5241, CNRS and Universite P. Sabatier, CHU Rangueil, Toulouse, France Abstract Background: Oxidative stress (OS), through excessive and/or chronic reactive oxygen species (ROS), is a mediator of diabetes-related damages in various tissues including pancreatic b-cells. Here, we have evaluated islet OS status and b-cell response to ROS using the GK/Par rat as a model of type 2 diabetes. Methodology/Principal Findings: Localization of OS markers was performed on whole pancreases. Using islets isolated from 7-day-old or 2.5-month-old male GK/Par and Wistar control rats, 1) gene expression was analyzed by qRT-PCR; 2) insulin secretion rate was measured; 3) ROS accumulation and mitochond