diagnostic accuracy of pcr in gambiense sleeping sickness diagnosis, staging and post-treatment follow-up a 2-year longitudinal studypcr在gambiense昏睡病诊断的准确率,分期和治疗后随访2年的纵向研究.pdfVIP

Diagnostic Accuracy of PCR in gambiense Sleeping Sickness Diagnosis, Staging and Post-Treatment Follow-Up: A 2-year Longitudinal Study 1,2. 1 . 1,3 ´ 1,3 Stijn Deborggraeve , Veerle Lejon * , Rosine Ali Ekangu , Dieudonne Mumba Ngoyi , Patient Pati 1,3 ´ 4 4 ¨ 1 Pyana , Medard Ilunga , Jean Pierre Mulunda , Philippe Buscher 1 Department of Parasitology, Institute of Tropical Medicine, Antwerp, Belgium, 2 Rega Institute, Catholic University of Leuven, Leuven, Belgium, 3 Department of ´ Parasitology, Institut National de Recherche Biomedicale, Kinshasa, Democratic Republic of the Congo, 4 Programme National de Lutte contre la Trypanosomiase Humaine Africaine, Mbuji-Mayi, Democratic Republic of the Congo Abstract Background: The polymerase chain reaction (PCR) has been proposed for diagnosis, staging and post-treatment follow-up of sleeping sickness but no large-scale clinical evaluations of its diagnostic accuracy have taken place yet. Methodology/Principal Findings: An 18S ribosomal RNA gene targeting PCR was performed on blood and cerebrospinal fluid (CSF) of 360 T. brucei gambiense sleeping sickness patients and on blood of 129 endemic controls from the Democratic Republic of Congo. Sensitivity and specificity (with 95% confidence intervals) of PCR for diagnosis, disease staging and treatment failure over 2 years follow-up post-treatment were determined. Reference standard tests were trypanosome detection for diagnosis and trypanosome detection and/or increased white blood cell concentration in CSF fo