differential glioma-associated tumor antigen expression profiles of human glioma cells grown in hypoxia微分glioma-associated肿瘤抗原的表达谱人类神经胶质瘤细胞生长在缺氧.pdfVIP

Differential Glioma-Associated Tumor Antigen Expression Profiles of Human Glioma Cells Grown in Hypoxia 1 2 1,3 1,3 3 Lisheng Ge , Andrew N. Cornforth , Neil T. Hoa , Christina Delgado , Shiun Kwei Chiou , Yi Hong Zhou4,5,6, Martin R. Jadus1,3,4,7* 1 Diagnostic and Molecular Health Care Group, Veterans Affairs Medical Center, Long Beach, California, United States of America, 2 California Stem Cell Inc., Irvine, California, United States of America, 3 Research Service Healthcare Group, Veterans Affairs Medical Center, Long Beach, California, United States of America, 4 Neuro- Oncology Program, Chao Comprehensive Cancer Center, University of California Irvine, Orange, California, United States of America, 5 Department of Neurological Surgery, University of California Irvine, Irvine, California, United States of America, 6 Department of Biological Chemistry, University of California Irvine, Irvine, California, United States of America, 7 Pathology and Laboratory Medicine, University of California Irvine, Irvine, California, United States of America Abstract Human U251 and D54 glioma cells were tested for expression of 25 glioma-associated tumor antigen precursor proteins (TAPP) under hypoxic (1% O ) or normoxic (21% O ) conditions. Hypoxic glioma cell lines increased their mRNA expression 2 2 for nine TAPP (Aim2, Art-4, EphA2, EZH2, Fosl1, PTH-rP, Sox 11, Whsc2 and YKL-40), as assessed by quantitative reverse transcriptase real-time/polymerase chain reaction (qRT-PCR). Increased differences with three hypoxic-induced TAPP: EZH2, Whsc2 and YKL-40 were shown at the protein levels by fluorescent antibody staining and quantitative e