differential expression of a-type and b-type lamins during hair cycling微分表达式a和b核纤层蛋白在头发骑自行车.pdfVIP

Differential Expression of A-Type and B-Type Lamins during Hair Cycling 1 2. 2. ¨ 3 2 Mubashir Hanif , Ylva Rosengardten , Hanna Sagelius , Bjorn Rozell , Maria Eriksson * ¨ 1The Folkhalsan Institute of Genetics, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland, 2 Department of Biosciences and Nutrition, Karolinska Institutet, Karolinska University Hospital, Huddinge, Novum, Stockholm, Sweden, 3 Clinical Research Center, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden Abstract Multiple genetic disorders caused by mutations that affect the proteins lamin A and C show strong skin phenotypes. These disorders include the premature aging disorders Hutchinson-Gilford progeria syndrome and mandibuloacral dysplasia, as well as restrictive dermopathy. Prior studies have shown that the lamin A/C and B proteins are expressed in skin, but little is known about their normal expression in the different skin cell-types and during the hair cycle. Our immunohistochemical staining for lamins A/C and B in wild-type mice revealed strong expression in the basal cell layer of the epidermis, the outer root sheath, and the dermal papilla during all stages of the hair cycle. Lower expression of both lamins A/C and B was seen in suprabasal cells of the epidermis, in the hypodermis, and in the bulb of catagen follicles. In addition, we have utilized a previously described mouse model of Hutchinson-Gilford progeria syndrome and show here that the expression of progerin does not result in pronounced effects on hair cycling or the expression of lamin B. Citation: Hanif M, Rosengardten Y, Sagelius H, Rozell B, Eriksson M (2009) Differe