differential impact of the hen1 homolog henn-1 on 21u and 26g rnas in the germline of caenorhabditis elegans微分的影响hen1同族体henn-1 u和26日21日g rna生殖系的线虫.pdfVIP

Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans 1.¤a 1.¤b 1. 1 Leonie M. Kamminga , Josien C. van Wolfswinkel , Maartje J. Luteijn , Lucas J. T. Kaaij , 2 2 2 1 ´ 1 Marloes P. Bagijn , Alexandra Sapetschnig , Eric A. Miska , Eugene Berezikov , Rene F. Ketting * 1 Hubrecht Institute–KNAW and University Medical Centre Utrecht, Utrecht, The Netherlands, 2 Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, United Kingdom Abstract RNA interference (RNAi)–related pathways affect gene activity by sequence-specific recruitment of Ago proteins to mRNA target molecules. The sequence specificity of this process stems from small RNA (sRNA) co-factors bound by the Ago protein. Stability of sRNA molecules in some pathways is in part regulated by Hen1-mediated methylation of their 39 ends. Here we describe the effects of the Caenorhabditis elegans HEN1 RNA–methyl-transferase homolog, HENN-1, on the different RNAi pathways in this nematode. We reveal differential effects of HENN-1 on the two pathways that are known to employ methylated sRNA molecules: the 26G and 21U pathways. Surprisingly, in the germline, stability of 21U RNAs, the C. elegans piRNAs, is only mildly affected by loss of methylation; and introduction of artificial 21U target RNA does not further destabilize non-methylated 21U RNAs. In contrast, most 26G RNAs display reduced stability and respond to loss of HENN-1 by displaying increased 39-uridylat