differential modulation of atp-induced p2x7-associated permeabilities to cations and anions of macrophages by infection with leishmania amazonensis微分调制atp-induced p2x7-associated渗透率阳离子和阴离子的巨噬细胞感染利什曼虫amazonensis.pdfVIP

Differential Modulation of ATP-Induced P2X7-Associated Permeabilities to Cations and Anions of Macrophages by Infection with Leishmania amazonensis 1 1 1 2 Camila Marques-da-Silva , Mariana Martins Chaves , Juliany Cola Rodrigues , Suzana Corte-Real , Robson Coutinho-Silva1*, Pedro Muanis Persechini 1 ˜ 1 Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil, 2 Instituto Oswaldo Cruz, Fundac¸ao Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil Abstract Leishmania and other parasites display several mechanisms to subvert host immune cell function in order to achieve successful infection. The ATP receptor P2X7, an agonist-gated cation channel widely expressed in macrophages and other cells of the immune system, is also coupled to inflammasome activation, IL-1 beta secretion, production of reactive oxygen species, cell death and the induction of the permeabilization of the plasma membrane to molecules of up to 900 Da. P2X7 receptors can function as an effective microbicidal triggering receptor in macrophages infected with several microorganisms including Mycobacteria tuberculosis, Chlamydia and Leishmania. We have previously shown that its expression is up-regulated in macrophages infected with L.amazonensis and that infected cells also display an increase in P2X7-induced apoptosis and membrane permeabilization to some anionic fluoresce