differential recognition of p. falciparum var2csa domains by naturally acquired antibodies in pregnant women from a malaria endemic area恶性疟原虫var2csa域的微分识别自然获得的抗体在孕妇疟疾流行区.pdfVIP

Differential Recognition of P. falciparum VAR2CSA Domains by Naturally Acquired Antibodies in Pregnant Women from a Malaria Endemic Area 1,2 1,2 1,2 3 1,2,4,5 Kim J. M. Brolin , Kristina E. M. Persson , Mats Wahlgren , Stephen J. Rogerson *, Qijun Chen * 1 Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden, 2 Department of Parasitology, Swedish Institute for Infectious Disease Control (SMI), Stockholm, Sweden, 3 Department of Medicine (RMH/WH), The University of Melbourne, Melbourne, Victoria, Australia, 4 Key Laboratory of Zoonosis, Ministry of Education and Jilin University, Changchun, China, 5 Institute of Pathogen Biology, Chinese Academy of Medical Science, Beijing, China Abstract Background: Plasmodium falciparum infected red blood cells (iRBC) express variant surface antigens (VSA) of which VAR2CSA is involved in placental sequestration and causes pregnancy-associated malaria (PAM). Primigravidae are most susceptible to PAM whereas antibodies associated with protection are often present at higher levels in multigravid women. However, HIV co-infection with malaria has been shown to alter this parity-dependent acquisition of immunity, with more severe symptoms as well as more malaria episodes in HIV positive women versus HIV negative women of a similar parity. Methods: Using VAR2CSA DBL-domains expressed on the surface of CHO-745 cells we quantified levels of DBL-domain specific IgG in sera from pregnant Malawian women by flow cytometry. Dissociations constants of DBL5e specific antibodies were determined using a surface plasmon resonance technique, as an indication of antibody