differential regulation of horizontally acquired and core genome genes by the bacterial modulator h-ns微分调节的水平和核心基因组基因的细菌调制器h-ns.pdfVIP

Differential Regulation of Horizontally Acquired and Core Genome Genes by the Bacterial Modulator H-NS ˜ 1. 2. 2 ´ ´ 3 3,4 2 Rosa C. Banos , Aitziber Vivero , Sonia Aznar , Jesus Garcıa , Miquel Pons , Cristina Madrid *, ´ 1,2* Antonio Juarez ´ 1 Institut de Bioenginyeria de Catalunya (IBEC), Parc Cientıfic de Barcelona, Barcelona, Spain, 2 Departament de Microbiologia, Facultat de Biologia, Universitat de ´ ´ ` Barcelona, Barcelona, Spain, 3 Institute for Research in Biomedicine (IRB Barcelona), Parc Cientıfic de Barcelona, Barcelona, Spain, 4 Departament de Quımica Organica, Universitat de Barcelona, Barcelona, Spain Abstract Horizontal acquisition of DNA by bacteria dramatically increases genetic diversity and hence successful bacterial colonization of several niches, including the human host. A relevant issue is how this newly acquired DNA interacts and integrates in the regulatory networks of the bacterial cell. The global modulator H-NS targets both core genome and HGT genes and silences gene expression in response to external stimuli such as osmolarity and temperature. Here we provide evidence that H-NS discriminates and differentially modulates core and HGT DNA. As an example of this, plasmid R27- encoded H-NS protein has evolved to selectively silence HGT genes and does not interfere with core genome regulation. In turn, differential regulation of b