direct effects of hiv-1 tat on excitability and survival of primary dorsal root ganglion neurons possible contribution to hiv-1-associated pain直接影响hiv - 1乙的主要背根神经节神经元的兴奋性和生存hiv-1-associated疼痛可能的贡献.pdfVIP

Direct Effects of HIV-1 Tat on Excitability and Survival of Primary Dorsal Root Ganglion Neurons: Possible Contribution to HIV-1-Associated Pain 1. 2. 2 3 3 2 Xianxun Chi , Tohti Amet , Daniel Byrd , Kuei-Hua Chang , Kavita Shah , Ningjie Hu , Ayslinn 2 2 1 4 1 2 Grantham , Sishun Hu , Jianhong Duan , Feng Tao , Grant Nicol , Qigui Yu * 1 Department of Pharmacology and Toxicology, Indiana University School of Medicine, Indianapolis, Indiana, United States of America, 2 Center for AIDS Research and Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, United States of America, 3 Department of Chemistry and Purdue University Center for Cancer Research, Purdue University, West Lafayette, Indiana, United States of America, 4 Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America Abstract The vast majority of people living with human immunodeficiency virus type 1 (HIV-1) have pain syndrome, which has a significant impact on their quality of life. The underlying causes of HIV-1-associated pain are not likely attributable to direct viral infection of the nervous system due to the lack of evidence of neuronal infection by HIV-1. However, HIV-1 proteins are possibly involved as they have been implicated in neuronal damage and death. The current study assesses the direct effects of HIV-1 Tat, one of potent neurotoxic viral proteins released from HIV-1-infected cells, on the excitability and survival of rat primary dorsal root ganglion (DRG) neurons. We demonstrated that HIV-1 Tat triggered rapid and sustained enhancement