dissecting interferon-induced transcriptional programs in human peripheral blood cells在人类外周血细胞解剖干扰素诱导转录项目.pdfVIP

Dissecting Interferon-Induced Transcriptional Programs in Human Peripheral Blood Cells 1 2 2,3 1 3,4 Simon J. Waddell *, Stephen J. Popper , Kathleen H. Rubins , Michael J. Griffiths , Patrick O. Brown , Michael Levin5, David A. Relman1,2,6 1 Department of Medicine, Stanford University, Stanford, California, United States of America, 2 Department of Microbiology and Immunology, Stanford University, Stanford, California, United States of America, 3 Department of Biochemistry, Stanford University, Stanford, California, United States of America, 4 Howard Hughes Medical Institute, Stanford University, Stanford, California, United States of America, 5 Department of Paediatrics, Imperial College London, London, United Kingdom, 6 Veterans Affairs Palo Alto Health Care System, Palo Alto, California, United States of America Abstract Interferons are key modulators of the immune system, and are central to the control of many diseases. The response of immune cells to stimuli in complex populations is the product of direct and indirect effects, and of homotypic and heterotypic cell interactions. Dissecting the global transcriptional profiles of immune cell populations may provide insights into this regulatory interplay. The host transcriptional response may also be useful in discriminating between disease states, and in understanding pathophysiology. The transcriptional programs of cell populations in health therefore provide a paradigm for deconvoluting disease-associated gene expression profiles. We used human cDNA microarrays to (1) compare the gene expression programs in human peripheral blood mononuclear cells (PBMCs) elicited by 6