distinct differences in chromatin structure at subtelomeric x and y elements in budding yeast不同的染色质结构的差异在subtelomeric x和y出芽酵母的元素.pdfVIP

Distinct Differences in Chromatin Structure at Subtelomeric X and Y’ Elements in Budding Yeast 1 1,2 Xuefeng Zhu *, Claes M. Gustafsson ¨ 1 Department of Biochemistry and Cell Biology, University of Gothenburg, Gothenburg, Sweden, 2 Max-Planck-Institut fur Biologie des Alterns, Cologne, Germany Abstract In Saccharomyces cerevisiae, all ends of telomeric DNA contain telomeric repeats of (TG1–3), but the number and position of subtelomeric X and Y’ repeat elements vary. Using chromatin immunoprecipitation and genome-wide analyses, we here demonstrate that the subtelomeric X and Y’ elements have distinct structural and functional properties. Y’ elements are transcriptionally active and highly enriched in nucleosomes, whereas X elements are repressed and devoid of nucleosomes. In contrast to X elements, the Y’ elements also lack the classical hallmarks of heterochromatin, such as high Sir3 and Rap1 occupancy as well as low levels of histone H4 lysine 16 acetylation. Our analyses suggest that the presence of X and Y’ elements govern chromatin structure and transcription activity at individual chromosome ends. Citation: Zhu X, Gustafsson CM (2009) Distinct Differences in Chromatin Structure at Subtelomeric X and Y’ Elements in Budding Yeast. PLoS ONE 4(7): e6363. doi:10.1371/journal.pone.0006363 Editor: Axel Imhof, University of Munich and Center of Integrated Protein Science, Germany Received January 12, 2009; Accepted June 21, 2009; Published July 23, 2009 Copyright: 2009 Zhu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unr