dose-dependent onset of regenerative program in neutron irradiated mouse skin存在剂量依赖的相关性在中子辐照小鼠皮肤出现再生项目.pdfVIP

Dose-Dependent Onset of Regenerative Program in Neutron Irradiated Mouse Skin 1. 2. 1 1 3 Emiliano Fratini , Valerio Licursi , Mara Artibani , Katarzyna Kobos , Paolo Colautti , Rodolfo 2,4 1 Negri , Roberto Amendola * ` 1 ENEA, Agenzia nazionale per le nuove tecnologie, l9energia e lo sviluppo economico sostenibile Roma, Italy, 2 Sapienza Universita di Roma, Dipartimento di Biologia e Biotecnologie ‘‘Charles Darwin’’, Roma, Italy, 3 INFN, Laboratori Nazionali di Legnaro, Legnaro, Italy, 4 Istituto Pasteur-Fondazione Cenci Bolognetti, Roma, Italy Abstract Background: Tissue response to irradiation is not easily recapitulated by cell culture studies. The objective of this investigation was to characterize, the transcriptional response and the onset of regenerative processes in mouse skin irradiated with different doses of fast neutrons. Methodology/Principal Findings: To monitor general response to irradiation and individual animal to animal variation, we performed gene and protein expression analysis with both pooled and individual mouse samples. A high-throughput gene expression analysis, by DNA oligonucleotide microarray was done with three months old C57Bl/6 mice irradiated with 0.2 and 1 Gy of mono-energetic 14 MeV neutron compared to sham irradiated controls. The results on 440 irradiation modulated genes, partially validated by quantitative real time RT-PCR, showed a dose-dependent up-regulation of a sub- class of keratin and keratin associated proteins, and m