dosage-sensitive function of retinoblastoma related and convergent epigenetic control are required during the arabidopsis life cycledosage-sensitive视网膜母细胞瘤相关的函数和收敛的表观遗传控制需要在拟南芥中生命周期.pdfVIP

Dosage-Sensitive Function of RETINOBLASTOMA RELATED and Convergent Epigenetic Control Are Required during the Arabidopsis Life Cycle 1,2 3 3¤a 2 3 Amal J. Johnston , Olga Kirioukhova , Philippa J. Barrell , Twan Rutten , James M. Moore , 3¤b 3 1 Ramamurthy Baskar , Ueli Grossniklaus , Wilhelm Gruissem * 1 Department of Biology and Zurich-Basel Plant Science Center, ETH Zurich, Zurich, Switzerland, 2 Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany, 3 Institute of Plant Biology and Zurich-Basel Plant Science Center, University of Zurich, Zurich, Switzerland Abstract The plant life cycle alternates between two distinct multi-cellular generations, the reduced gametophytes and the dominant sporophyte. Little is known about how generation-specific cell fate, differentiation, and development are controlled by the core regulators of the cell cycle. In Arabidopsis, RETINOBLASTOMA RELATED (RBR), an evolutionarily ancient cell cycle regulator, controls cell proliferation, differentiation, and regulation of a subset of Polycomb Repressive Complex 2 (PRC2) genes and METHYLTRANSFERASE 1 (MET1) in the male and female gametophytes, as well as cell fate establishment in the male gametophyte. Here we demonstrate that RBR is also essential for cell fate determination in the female gametophyte, as revealed by loss of cell-specific marker expression in all the gametophytic cells that lack RBR. Maintenance of genome integrity also requires RBR, because diploid plants heterozygous for rbr (rbr/RBR) produce an abnormal portion of triploi