down-regulated nod2 by immunosuppressants in peripheral blood cells in patients with sle reduces the muramyl dipeptide-induced il-10 production理气nod2免疫抑制剂在系统性红斑狼疮患者外周血细胞减少胞壁dipeptide-induced il - 10的生产.pdfVIP

Down-Regulated NOD2 by Immunosuppressants in Peripheral Blood Cells in Patients with SLE Reduces the Muramyl Dipeptide-Induced IL-10 Production 1 2 2 2,3 1 Shui-Lian Yu , Chun-Kwok Wong , Purple Tsz-Yan Wong , Da-Peng Chen , Cheuk-Chun Szeto , 1 1 Edmund K. Li , Lai-Shan Tam * 1 Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, China, 2 Department of Chemical Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, China, 3 Department of Clinical Laboratory, Children’s Hospital of Chongqing Medical University, Chongqing, China Abstract Background: Pattern recognition receptors (PRRs) such as Toll-like receptors are aberrantly expressed of peripheral blood mononuclear cells (PBMCs) in systemic lupus erythematosus (SLE) patients, for playing immunopathological roles. Methodology/Principal Findings: We investigated the expression and function of the PRR nucleotide-binding oligomerization domain (NOD2) in SLE. NOD2 expression in T, B lymphocytes, monocytes, myeloid dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs) was assessed in SLE patients and healthy controls (HCs) using flow cytometric analysis. Ex vivo production of cytokines from PBMCs upon NOD2 agonist muramyl dipeptide (MDP) stimulation was ¨ assessed using Cytometric B