down-regulation of mir-92 in human plasma is a novel marker for acute leukemia patients下调在人血浆mir - 92是一种新型标记为急性白血病患者.pdfVIP

Down-Regulation of miR-92 in Human Plasma Is a Novel Marker for Acute Leukemia Patients 1 1,2¤ 1,2 1 3 Masami Tanaka , Kosuke Oikawa , Masakatsu Takanashi , Motoshige Kudo , Junko Ohyashiki , 4 1 Kazuma Ohyashiki , Masahiko Kuroda * 1 Department of Pathology, Tokyo Medical University, Tokyo, Japan, 2 Department of Cell Therapy, Tokyo Medical University, Tokyo, Japan, 3 Intractable Disease Therapeutic Research Center, Tokyo Medical University, Tokyo, Japan, 4 First Department of Internal Medicine, Tokyo Medical University, Tokyo, Japan Abstract Background: MicroRNAs are a family of 19- to 25-nucleotides noncoding small RNAs that primarily function as gene regulators. Aberrant microRNA expression has been described for several human malignancies, and this new class of small regulatory RNAs has both oncogenic and tumor suppressor functions. Despite this knowledge, there is little information regarding microRNAs in plasma especially because microRNAs in plasma, if exist, were thought to be digested by RNase. Recent studies, however, have revealed that microRNAs exist and escape digestion in plasma. Methodology/Principal Findings: We performed microRNA microaray to obtain insight into microRNA deregulation in the plasma of a leukemia patient. We have revealed that microRNA-638 (miR-638) is stably present in human plasmas, and microRNA-92a (miR-92a) dramatically decreased in the plasmas of acute leukemia patients. Especially, the ratio of miR-92a/ miR-638 in plasma was very useful for distinguishing leukemia patients from healthy body. Conclusions/Significance: The ratio of miR-92a/miR-638 in plasm