drw201p65 tetramer visualization of epitope-specific cd4 t-cell during m. tuberculosis infection and its resting memory pool after bcg vaccinationdrw201p65四聚物的可视化epitope-specific cd4 t细胞在结核分枝杆菌感染及其接种卡介苗后休息内存池.pdfVIP

DR*W201/P65 Tetramer Visualization of Epitope-Specific CD4 T-Cell during M. tuberculosis Infection and Its Resting Memory Pool after BCG Vaccination 1¤ 1 1 1 1 2 Huiyong Wei , Richard Wang , Zhuqing Yuan , Crystal Y. Chen , Dan Huang , Lisa Halliday , Weihua 1 1 1 1 1 1 Zhong , Gucheng Zeng , Yun Shen , Ling Shen , Yunqi Wang , Zheng W. Chen * 1 Department of Immunology Microbiology, Center for Primate Biomedical Research, University of Illinois College of Medicine at Chicago (UIC), Chicago, Illinois, United States of America, 2 Biological Resource Laboratory, University of Illinois at Chicago (UIC), Chicago, Illinois, United States of America Abstract Background: In vivo kinetics and frequencies of epitope-specific CD4 T cells in lymphoid compartments during M. tuberculosis infection and their resting memory pool after BCG vaccination remain unknown. Methodology/Findings: Macaque DR*W201 tetramer loaded with Ag85B peptide 65 was developed to directly measure epitope-specific CD4 T cells in blood and tissues form macaques after M. tuberculosis infection or BCG vaccination via direct staining and tetramer-enriched approach. The tetramer-based enrichment approach showed that P65 epitope-specific CD4 T cells emerged at mean frequencies of ,500 and ,4500 per 107 PBL at days 28 and 42, respectively, and at day 63 increased further to ,22,000/107 PBL after M. tuberculosis infection. Direct tetramer staining showed that the tetramer- bound P65-specific T cells constituted about 0.2–0.3% o