drosophila tan encodes a novel hydrolase required in pigmentation and vision果蝇棕褐色色素沉着中所需编码一种水解酶和愿景.pdfVIP

Drosophila tan Encodes a Novel Hydrolase Required in Pigmentation and Vision 1* 1 2 2 3 4 John R. True , Shu-Dan Yeh , Bernhard T. Hovemann , Tobias Kemme , Ian A. Meinertzhagen , Tara N. Edwards , 1 5 5 Shian-Ren Liou , Qian Han , Jianyong Li ¨ ¨ ¨ 1 Department of Ecology and Evolution, State University of New York, Stony Brook, New York, United States of America, 2 Fakultat fur Chemie, Ruhr-Universitat Bochum, Bochum, Germany, 3 Life Sciences Centre, Dalhousie University, Halifax, Nova Scotia, Canada, 4 Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada, 5 Department of Veterinary Pathology, College of Veterinary Medicine, University of Illinois, Urbana, Illinois, United States of America Many proteins are used repeatedly in development, but usually the function of the protein is similar in the different contexts. Here we report that the classical Drosophila melanogaster locus tan encodes a novel enzyme required for two very different cellular functions: hydrolysis of N-b-alanyl dopamine (NBAD) to dopamine during cuticular melanization, and hydrolysis of carcinine to histamine in the metabolism of photoreceptor neurotransmitter. We characterized two tan-like P-element insertions that failed to complement classical tan mutations. Both are inserted in the 59 untranslated region of the previously uncharacterized gene CG12120, a putative homolog of fungal isopenicillin-N N-acyltransferase (EC 64). Both P insertions showed abnormally low transcription of the CG12120 mRNA. Ectopic CG12120 expression rescued tan mutant pigmentation