divergence of the yeast transcription factor fzf1 affects sulfite resistance散度的酵母转录因子fzf1影响亚硫酸盐阻力.pdfVIP

Divergence of the Yeast Transcription Factor FZF1 Affects Sulfite Resistance 1 2 Elizabeth K. Engle , Justin C. Fay * 1 Molecular Genetics and Genomics Program, Washington University, St. Louis, Missouri, United States of America, 2 Department of Genetics and Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, Missouri, United States of America Abstract Changes in gene expression are commonly observed during evolution. However, the phenotypic consequences of expression divergence are frequently unknown and difficult to measure. Transcriptional regulators provide a mechanism by which phenotypic divergence can occur through multiple, coordinated changes in gene expression during development or in response to environmental changes. Yet, some changes in transcriptional regulators may be constrained by their pleiotropic effects on gene expression. Here, we use a genome-wide screen for promoters that are likely to have diverged in function and identify a yeast transcription factor, FZF1, that has evolved substantial differences in its ability to confer resistance to sulfites. Chimeric alleles from four Saccharomyces species show that divergence in FZF1 activity is due to changes in both its coding and upstream noncoding sequence. Between the two closest species, noncoding changes affect the expression of FZF1, whereas coding changes affect the expression of SSU1, a sulfite efflux pump activated by FZF1. Both coding and noncoding changes also affect the expression of many other genes. Our results show how divergence in the coding and promoter region of a transcription factor alters the response to an environmental stress. Citation: Engle EK, Fay JC (2012) Divergence of the Yeast Transcri