diurnally entrained anticipatory behavior in archaea每日携入的先行行为古生菌.pdfVIP

Diurnally Entrained Anticipatory Behavior in Archaea 1 1 2 3 1,4 Kenia Whitehead , Min Pan , Ken-ichi Masumura , Richard Bonneau , Nitin S. Baliga * 1 Institute for Systems Biology, Seattle, Washington, United States of America, 2 Division of Genetics and Mutagenesis, National Institute of Health Science, Tokyo, Japan, 3 Center for Genomics and Systems Biology, Courant Institute of Mathematical Science, Department of Computer Science, New York University, New York, New York, United States of America, 4 Department of Microbiology and Molecular and Cellular Biology, University of Washington, Seattle, Washington, United States of America Abstract By sensing changes in one or few environmental factors biological systems can anticipate future changes in multiple factors over a wide range of time scales (daily to seasonal). This anticipatory behavior is important to the fitness of diverse species, and in context of the diurnal cycle it is overall typical of eukaryotes and some photoautotrophic bacteria but is yet to be observed in archaea. Here, we report the first observation of light-dark (LD)-entrained diurnal oscillatory transcription in up to 12% of all genes of a halophilic archaeon Halobacterium salinarum NRC-1. Significantly, the diurnally entrained transcription was observed under constant darkness after removal of the LD stimulus (free-running rhythms). The memory of diurnal entrainment was also associated with the synchronization of oxic and anoxic physiologies to the LD cycle. Our results suggest that under nutrient limited conditions halophilic archaea take advantage of the causal influence of sunlight (via temperature) on O2 diffusivity in a closed hypersaline environment