ectopic expression reveals a conserved phyb homolog in soybean在大豆异位表达揭示了一个守恒、同族体.pdfVIP

Ectopic Expression Reveals a Conserved PHYB Homolog in Soybean Fa-Qiang Wu, Xiao-Mei Zhang, Dong-Mei Li, Yong-Fu Fu* Institute of Crop Sciences, National Key Facility of Crop Gene Resource and Genetic Improvement, Chinese Academy of Agricultural Sciences, Haidian District, Beijing, China Abstract Phytochromes sense red/far-red light and trigger a cascade of physiological responses in plant. Here, a phytochrome B homolog, GmPHYB1, was amplified from the soybean genome, and its expression profiles were obtained for various parts of the plant and at various developmental stages. The gene was ectopically expressed in Arabidopsis thaliana, driven by CaMV 35S promoter, to study the physiological functions of the gene product. The overexpressors of GmPHYB1 behaved similarly to those of AtPHYB, but with some subtle differences with respect to the acceleration of flowering under short day conditions and the growth of the hypocotyl under certain light fluence rate. The results suggested that this soybean PHYB homolog was well conserved both at the level of sequence and physiological function. Citation: Wu F-Q, Zhang X-M, Li D-M, Fu Y-F (2011) Ectopic Expression Reveals a Conserved PHYB Homolog in Soybean. PLoS ONE 6(11): e27737. doi:10.1371/ journal.pone.0027737 ¨ ˚ Editor: Par K. Ingvarsson, University of Umea, Sweden Received August 3, 2011; Accepted October 24, 2011; Published November 16, 2011 Copyright: 2011 Wu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work was partly supported by Trans