down-regulation of small rubber particle protein expression affects integrity of rubber particles and rubber content in taraxacum brevicorniculatum下调小橡胶粒子蛋白表达影响橡胶颗粒的完整性和橡胶含量蒲公英brevicorniculatum.pdfVIP

Down-Regulation of Small Rubber Particle Protein Expression Affects Integrity of Rubber Particles and Rubber Content in Taraxacum brevicorniculatum 1,3 1 1 1 1 Andrea Hillebrand , Janina J. Post , David Wurbs , Daniela Wahler , Malte Lenders , 2,4 ¨ 1,3 3 Vladislav Krzyzanek , Dirk Prufer , Christian Schulze Gronover * ¨ 1 Institute of Plant Biology and Biotechnology, Westphalian Wilhelms-University, Munster, Germany, 2 Institute for Medical Physics and Biophysics, Westphalian Wilhelms- ¨ University, Munster, Germany, 3 Fraunhofer Institute for Molecular Biology and Applied Ecology, Aachen, Germany, 4 Institute of Scientific Instruments of the ASCR, v.v.i., Academy of Sciences of the Czech Republic, Brno, Czech Republic Abstract The biosynthesis of rubber is thought to take place on the surface of rubber particles in laticifers, highly specialized cells that are present in more than 40 plant families. The small rubber particle protein (SRPP) has been supposed to be involved in rubber biosynthesis, and recently five SRPPs (TbSRPP1–5) were identified in the rubber-producing dandelion species Taraxacum brevicorniculatum. Here, we demonstrate by immunogold labeling that TbSRPPs are localized to rubber particles, and that rubber particles mainly consist of TbSRPP3, 4 and 5 as shown by high-resolution two-dimensional gel electrophoresis and mass spectrometric analysis. We also carried out an RNA-interference approach in transgenic plants to address the function of TbSRPPs in rubber biosynth