down-regulation of yes associated protein 1 expression reduces cell proliferation and clonogenicity of pancreatic cancer cells是的相关蛋白1表达的下调减少细胞增殖和clonogenicity胰腺癌细胞.pdfVIP

Down-Regulation of Yes Associated Protein 1 Expression Reduces Cell Proliferation and Clonogenicity of Pancreatic Cancer Cells 1 1 2 2 3 Caroline H. Diep , Kelly M. Zucker , Galen Hostetter , Aprill Watanabe , Chengcheng Hu , Ruben M. 1 1 1 Munoz , Daniel D. Von Hoff , Haiyong Han * 1 Clinical Translational Division, Translational Genomics Research Institute, Scottsdale, Arizona, United States of America, 2 Integrated Cancer Genomics Division, Translational Genomics Research Institute, Scottsdale, Arizona, United States of America, 3 Epidemiology and Biostatistics Division, College of Public Health, University of Arizona, Tucson, Arizona, United States of America Abstract Background: The Hippo pathway regulates organ size by inhibiting cell proliferation and promoting cell apoptosis upon its activation. The Yes Associated Protein 1 (YAP1) is a nuclear effector of the Hippo pathway that promotes cell growth as a transcription co-activator. In human cancer, the YAP1 gene was reported as amplified and over-expressed in several tumor types. Methods: Immunohistochemical staining of YAP1 protein was used to assess the expression of YAP1 in pancreatic tumor tissues. siRNA oligonucleotides were used to knockdown the expression of YAP1 and their effects on pancreatic cancer cells were investigated using cell proliferation, apoptosis, and anchorage-independent growth assays. The Wilcoxon signed-rank, Pearson correlation coefficient, Kendall’s Tau, Spearman’s Rho, and an independent two-sample t (two-tailed) test were used to determine the statistical significance of the data. Results: Immunohistochemistry studies in pancreatic tumor tissues revealed YAP1 staining intensities were moderate to