dissemination of clonal groups of brachyspira hyodysenteriae amongst pig farms in spain, and their relationships to isolates from other countries传播克隆组brachyspira hyodysenteriae在养猪场在西班牙,隔离来自其他国家和他们的关系.pdfVIP

Dissemination of Clonal Groups of Brachyspira hyodysenteriae amongst Pig Farms in Spain, and Their Relationships to Isolates from Other Countries ´ 1 1 ´ 1 2 2 1 Jesus Osorio , Ana Carvajal , German Naharro , Tom La , Nyree D. Phillips , Pedro Rubio , David J. Hampson2* ´ ´ 1 Animal Health Department, Faculty of Veterinary Science, University of Leon, Leon, Spain, 2 School of Veterinary and Biomedical Science, Murdoch University, Murdoch, Western Australia, Australia Abstract Background: Swine dysentery (SD) is a widespread diarrhoeal disease of pigs caused by infection of the large intestine with the anaerobic intestinal spirochaete Brachyspira hyodysenteriae. Understanding the dynamics of SD, and hence being able to develop more effective measures to counter its spread, depends on the ability to characterise B. hyodysenteriae variants and trace relationships of epidemic strains. Methodology/Principal Findings: A collection of 51 Spanish and 1 Portuguese B. hyodysenteriae isolates was examined using a multilocus sequence typing (MLST) scheme based on the sequences of seven conserved genomic loci. The isolates were allocated to 10 sequence types (STs) in three major groups of descent. Isolates in four of the STs were widely distributed in farms around Spain. One farm was infected with isolates from more than one ST. Sequence data obtained from PubMLST for 111 other B. hyodysenteriae strains from other countries then were included in the analysis. Two of the predominant STs that were found in Spain also were present in other European countries. The