down-regulation of replication factor c-40 (rfc40) causes chromosomal missegregation in neonatal and hypertrophic adult rat cardiac myocytes下调的复制因子c-40(rfc40)导致新生儿和肥厚性染色体missegregation成年老鼠心脏细胞.pdfVIP

Down-Regulation of Replication Factor C-40 (RFC40) Causes Chromosomal Missegregation in Neonatal and Hypertrophic Adult Rat Cardiac Myocytes 1. 2. 3 1 3 4 Hirotaka Ata , Deepa Shrestha , Masahiko Oka , Rikuo Ochi , Chian Ju Jong , Sarah Gebb , 5 3 2¤ 6 2 1 John Benjamin , Stephen Schaffer , Holly H. Hobart , James Downey , Ivan McMurtry , Rakhee Gupte * 1 Biochemistry Molecular Biology, University of South Alabama, Mobile, Alabama, United States of America, 2 Pathology, University of South Alabama, Mobile, Alabama, United States of America, 3 Pharmacology Center for Lung Biology, University of South Alabama, Mobile, Alabama, United States of America, 4 Cell Biology Neuroscience, University of South Alabama, Mobile, Alabama, United States of America, 5 Pediatrics and Neonatology, University of South Alabama, Mobile, Alabama, United States of America, 6 Physiology, University of South Alabama, Mobile, Alabama, United States of America Abstract Background: Adult mammalian cardiac myocytes are generally assumed to be terminally differentiated; nonetheless, a small fraction of cardiac myocytes have been shown to replicate during ventricular remodeling. However, the expression of Replication Factor C (RFC; RFC140/40/38/37/36) and DNA polymerase d (Pol d) proteins, which are required for DNA synthesis and cell proliferation, in the adult normal and hypertrophied hearts has been rarely studied. Methods: We performed qRT-PCR and Western blot analysis to determine the levels of RFC and Pol d message and proteins in the adult normal cardiac