downregulation of rpl6 by sirna inhibits proliferation and cell cycle progression of human gastric cancer cell linesdownregulation rpl6核的抑制增殖和人类胃癌细胞株的细胞周期进程.pdfVIP

Downregulation of RPL6 by siRNA Inhibits Proliferation and Cell Cycle Progression of Human Gastric Cancer Cell Lines 1. 1,2. 2 1 1 1 1 Qiong Wu , Yawen Gou , Qiaomin Wang , Haifeng Jin , Lina Cui , Yongguo Zhang , Lijie He , Jingbo 1 1 1 1 Wang , Yongzhan Nie , Yongquan Shi *, Daiming Fan * 1 State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, the Fourth Military Medical University, Xi’an, Shaanxi, China, 2 Department of Digestive Diseases, The Affiliated Provincial Hospital of Anhui Medical University, Hefei, Anhui Province, China Abstract Our previous study revealed that human ribosomal protein L6 (RPL6) was up-regulated in multidrug-resistant gastric cancer cells and over-expression of RPL6 could protect gastric cancer from drug-induced apoptosis. It was further demonstrated that up-regulation of RPL6 accelerated growth and enhanced in vitro colony forming ability of GES cells while down- regulation of RPL6 exhibited the opposite results. The present study was designed to investigate the potential role of RPL6 in therapy of gastric cancer for clinic. The expression of RPL6 and cyclin E in gastric cancer tissues and normal gastric mucosa was evaluated by immunohistochemisty. It was found that RPL6 and cyclin E were expressed at a higher level in gastric cancer tissues than that in normal gastric mucosa and the two were correlative in gastric cancer. Survival time of postoperative patients was analyzed by Kaplan- Meier analysis and it was found that patients with RPL6 positive expression showed shorter survival time than patients that with RPL6 negative expression. RPL6 was then genetically dow