edta improves stability of whole blood c-peptide and insulin to over 24 hours at room temperatureedta改善全血c -肽的稳定性和胰岛素在室温在24小时.pdfVIP

EDTA Improves Stability of Whole Blood C-Peptide and Insulin to Over 24 Hours at Room Temperature 1,2 . 1. 3 4 1 Timothy J. McDonald * , Mandy H. Perry , Roy W. A. Peake , Nicola J. Pullan , John O’Connor , 2 2 2 Beverley M. Shields , Beatrice A. Knight , Andrew T. Hattersley 1 Department of Clinical Chemistry, Royal Devon and Exeter NHS Foundation Trust, Exeter, United Kingdom, 2 NIHR Clinical Research Facility, Peninsula College of medicine and Dentistry, Barrack Road, University of Exeter, Exeter, United Kingdom, 3 Department of Clinical Biochemistry, NHS Grampian Health Board, Aberdeen, United Kingdom, 4 Clinical Pathology (City Campus), Nottingham University Hospitals NHS Trust, Hucknall Road, Nottingham, United Kingdom Abstract Introduction: C-peptide and insulin measurements in blood provide useful information regarding endogenous insulin secretion. Conflicting evidence on sample stability and handling procedures continue to limit the widespread clinical use of these tests. We assessed the factors that altered the stability of insulin and C-peptide in blood. Methods: We investigated the impact of preservative type, time to centrifugation, storage conditions and duration of storage on the stability of C-peptide and insulin on three different analytical platforms. Results: C-peptide was stable for at least 24 hours at room temperature in both centrifuged and whole blood collected in K+-EDTA and serum gel tubes, with the exception of whole blood serum gel, which decreased to 78% of baseline at 24 hours, (p = 0.008). Insulin was stable at room temperature for 24 hou