eimeria species and genetic background influence the serum protein profile of broilers with coccidiosis艾美球虫属物种和遗传背景影响血清蛋白的肉鸡球虫病.pdfVIP

Eimeria Species and Genetic Background Influence the Serum Protein Profile of Broilers with Coccidiosis 1 1 1 1 1 Elizabeth R. Gilbert , Chasity M. Cox , Patricia M. Williams , Audrey P. McElroy , Rami A. Dalloul , W. 2 1 3 1 1 Keith Ray , Adriana Barri , Derek A. Emmerson , Eric A. Wong , Kenneth E. Webb Jr. * 1 Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, Virginia, United States of America, 2 Department of Biochemistry, Virginia Tech, Blacksburg, Virginia, United States of America, 3 Aviagen, Huntsville, Alabama, United States of America Abstract Background: Coccidiosis is an intestinal disease caused by protozoal parasites of the genus Eimeria. Despite the advent of anti-coccidial drugs and vaccines, the disease continues to result in substantial annual economic losses to the poultry industry. There is still much unknown about the host response to infection and to date there are no reports of protein profiles in the blood of Eimeria-infected animals. The objective of this study was to evaluate the serum proteome of two genetic lines of broiler chickens after infection with one of three species of Eimeria. Methodology/Principal Findings: Birds from lines A and B were either not infected or inoculated with sporulated oocysts from one of the three Eimeria strains at 15 d post-hatch. At 21 d (6 d post-infection), whole blood was collected and lesion scoring was performed. Serum was harvested and used for 2-dimensional gel electrophoresis. A total of 1,266 spots were quantitatively assessed by densitometry. Protein spots showing a significa