electrostatic-assembly-driven formation of supramolecular rhombus microparticles and their application for fluorescent nucleic acid detectionelectrostatic-assembly-driven形成超分子菱形微粒子和荧光核酸检测的应用.pdfVIP

Electrostatic-Assembly-Driven Formation of Supramolecular Rhombus Microparticles and Their Application for Fluorescent Nucleic Acid Detection 1,2 1 1 Hailong Li , Junfeng Zhai , Xuping Sun * 1 State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, China, 2 Graduate School of the Chinese Academy of Sciences, Beijing, China Abstract In this paper, we report on the large-scale formation of supramolecular rhombus microparticles (SRMs) driven by electrostatic assembly, carried out by direct mixing of an aqueous HAuCl4 solution and an ethanol solution of 4,49-bipyridine at room temperature. We further demonstrate their use as an effective fluorescent sensing platform for nucleic acid detection with a high selectivity down to single-base mismatch. The general concept used in this approach is based on adsorption of the fluorescently labeled single-stranded DNA (ssDNA) probe by SRM, which is accompanied by substantial fluorescence quenching. In the following assay, specific hybridization with its target to form double-stranded DNA (dsDNA) results in desorption of ssDNA from SRM surface and subsequent fluorescence recovery. Citation: Li H, Zhai J, Sun X (2011) Electrostatic-Assembly-Driven Formation of Supramolecular Rhombus Microparticles and Their Application for Fluorescent Nucleic Acid Detection. PLoS ONE 6(4): e18958. doi:10.1371/journal.pone.0018958 Editor: Jian R. Lu, The University of Manchester, United Kingdom Received November 30, 2010; Accepted March 14, 2011; Published April 19, 2011 Copyright: 2011 Li et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any m