no evidence of murine leukemia virus-related viruses in live attenuated human vaccines没有证据表明小鼠白血病病毒相关病毒减毒活疫苗人类疫苗.pdfVIP

No Evidence of Murine Leukemia Virus-Related Viruses in Live Attenuated Human Vaccines 1 1 2 2 1 William M. Switzer *, HaoQiang Zheng , Graham Simmons , Yanchen Zhou , Shaohua Tang , Anupama 1 2 2 1 Shankar , Beatrix Kapusinszky , Eric L. Delwart , Walid Heneine 1 Laboratory Branch, Division of HIV/AIDS Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America, 2 Blood Systems Research Institute and Department of Laboratory Medicine, University of California (UCSF), San Francisco, San Francisco, California, United States of America Abstract Background: The association of xenotropic murine leukemia virus (MLV)-related virus (XMRV) in prostate cancer and chronic fatigue syndrome reported in previous studies remains controversial as these results have been questioned by recent data. Nonetheless, concerns have been raised regarding contamination of human vaccines as a possible source of introduction of XMRV and MLV into human populations. To address this possibility, we tested eight live attenuated human vaccines using generic PCR for XMRV and MLV sequences. Viral metagenomics using deep sequencing was also done to identify the possibility of other adventitious agents. Results: All eight live attenuated vaccines, including Japanese encephalitis virus (JEV) (SA-14-14-2), varicella (Varivax), measles, mumps, and rubella (MMR-II), measles (Attenuvax), rubella (Meruvax-II), rotavirus (Rotateq and Rotarix), and yellow fever virus were negative for XMRV and highly related MLV sequences. However, residual hamster DNA, but