non-coding changes cause sex-specific wing size differences between closely related species of nasonia非编码的变化导致性别翼nasonia密切相关的物种之间的大小差异.pdfVIP

Non-Coding Changes Cause Sex-Specific Wing Size Differences between Closely Related Species of Nasonia 1 1¤a 1 1¤b David W. Loehlin , Deodoro C. S. G. Oliveira , Rachel Edwards , Jonathan D. Giebel , Michael E. 1 1 2 2 2 Clark , M. Victoria Cattani , Louis van de Zande , Eveline C. Verhulst , Leo W. Beukeboom , Monica ˜ 3¤c 1 Munoz-Torres , John H. Werren * 1 Department of Biology, University of Rochester, Rochester, New York, United States of America, 2 Evolutionary Genetics, Centre for Ecological and Evolutionary Studies, University of Groningen, Haren, The Netherlands, 3 Clemson University Genomics Institute, Clemson University, Clemson, South Carolina, United States of America Abstract The genetic basis of morphological differences among species is still poorly understood. We investigated the genetic basis of sex-specific differences in wing size between two closely related species of Nasonia by positional cloning a major male- specific locus, wing-size1 (ws1). Male wing size increases by 45% through cell size and cell number changes when the ws1 allele from N. giraulti is backcrossed into a N. vitripennis genetic background. A positional cloning approach was used to fine-scale map the ws1 locus to a 13.5 kilobase region. This region falls between prospero (a transcription factor involved in neurogenesis) and the master sex-determining gene doublesex. It contains the 59-UTR and cis-regulatory domain of doublesex, and no coding sequence. Wing size reduction correlates with an in